Hotspot

rpl33-rps18

LSC · 71519-71866 · 348 bp

Back to Aristolochia

Pi 0.116

MarkerSeek score 72.80

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 348
variable_sites: 153
pi: 0.116
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	GCATACGATTCATGGGGAGA	GCAAACGCCTACGAAAAGAT	50.0	45.0	57.2	57.1	262-565 bp	1.0	78.1
rpl33-rps18_p2	GCATACGATTCATGGGGAGA	CGCTTGGATTTACGAAAGGG	50.0	50.0	57.2	57.2	242-545 bp	0.974359	77.7
rpl33-rps18_p3	AGCATACGATTCATGGGGAG	CGCTTGGATTTACGAAAGGG	50.0	50.0	57.2	57.2	243-546 bp	0.974359	77.6
rpl33-rps18_p4	GCATACGATTCATGGGGAGA	CGAAAGGGTCGCTTGGATTT	50.0	50.0	57.2	58.8	230-533 bp	0.948718	76.6
rpl33-rps18_p5	AGCATACGATTCATGGGGAG	CGAAAGGGTCGCTTGGATTT	50.0	50.0	57.2	58.8	231-534 bp	0.948718	76.5