Hotspot

petA-psbJ

LSC · 66806-67924 · 1119 bp

Back to Aristolochia

Pi 0.047

MarkerSeek score 81.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1119
variable_sites: 256
pi: 0.047
unique_haplotype_count: 37
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TCAGAGCTATCGCCCCAATA	TTAGGAGGCCTCAATGACCA	50.0	50.0	58.0	58.0	2168-2273 bp	0.948718	89.6
petA-psbJ_p2	TCAGAGCTATCGCCCCAATA	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	2219-2324 bp	0.871795	86.4
petA-psbJ_p3	TCAGAGCTATCGCCCCAATA	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	2218-2323 bp	0.871795	86.4
petA-psbJ_p4	TCCGATCGTATTTCTCCGGA	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	2287-2300 bp	0.128205	46.8
petA-psbJ_p5	TCCGATCGTATTTCTCCGGA	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	2286-2299 bp	0.128205	46.8