Hotspot

atpH-atpI

LSC · 13736-14986 · 1251 bp

Back to Aristolochia

Pi 0.0403

MarkerSeek score 78.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1251
variable_sites: 250
pi: 0.0403
unique_haplotype_count: 37
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CAAATTAGTCCTTCCCCGGG	GGCAAATAGGGGGTTTCCAA	55.0	50.0	57.9	58.1	2284-2448 bp	0.974359	90.1
atpH-atpI_p2	CAAATTAGTCCTTCCCCGGG	TTCGGAATCCACAAACCGTT	55.0	45.0	57.9	58.0	2194-2358 bp	0.948718	89.3
atpH-atpI_p3	AACAGAAGCGGCAGAAATCA	TAGGTTCAGCCGCTATAGCT	45.0	50.0	58.1	58.0	1836-2379 bp	0.948718	89.1
atpH-atpI_p4	CAAATTAGTCCTTCCCCGGG	TAGGTTCAGCCGCTATAGCT	55.0	50.0	57.9	58.0	2215-2379 bp	0.923077	88.5
atpH-atpI_p5	AAAGGCGGGAAGGAAGAAAG	TAGGTTCAGCCGCTATAGCT	50.0	50.0	58.1	58.0	2264-2387 bp	0.923077	88.2