Hotspot

matK-trnK-UUU

LSC · 3312-4140 · 829 bp

Back to Aristolochia

Pi 0.0515

MarkerSeek score 81.30

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 829
variable_sites: 172
pi: 0.0515
unique_haplotype_count: 37
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	GGGAGCATCTTGTATCCAGC	GGGCAATCCCGCTTATTGTA	55.0	50.0	58.1	58.0	1777-2659 bp	0.846154	84.8
matK-trnK-UUU_p2	AGAAAGGCTCCAGAGGATGT	GGGCAATCCCGCTTATTGTA	50.0	50.0	58.0	58.0	1989-2871 bp	0.794872	83.3
matK-trnK-UUU_p3	AGGCTCCAGAGGATGTTGAT	GGGCAATCCCGCTTATTGTA	50.0	50.0	58.1	58.0	1985-2867 bp	0.794872	82.7
matK-trnK-UUU_p4	TCAAGAAAGGCTCCAGAGGA	GGGCAATCCCGCTTATTGTA	50.0	50.0	58.0	58.0	1992-2874 bp	0.769231	82.3
matK-trnK-UUU_p5	TCAAGAAAGGCTCCAGAGGA	TCCGGGTTGCTAACTCAATG	50.0	50.0	58.0	57.9	1542-1965 bp	0.769231	80.3