Hotspot

rpl16

LSC · 77285-78709 · 1425 bp

Back to Aristida

Pi 0.0132

MarkerSeek score 57.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1425
variable_sites: 59
pi: 0.0132
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AATTGCGCCAAAAACTCGAG	GCAGACTCAAGTTCCGAAGT	45.0	50.0	57.9	57.8	2486-2504 bp	1.0	82.7
rpl16_p2	AATTGCGCCAAAAACTCGAG	TCCCATTCTGGCCAGTGATA	45.0	50.0	57.9	58.1	1979-1997 bp	0.875	77.4
rpl16_p3	TCAGCAATAGTGTCCTTGCC	TCCCATTCTGGCCAGTGATA	50.0	50.0	57.9	58.1	2205-2230 bp	0.875	76.8
rpl16_p4	AATTGCGCCAAAAACTCGAG	AAATTGCAGGGCGTTTAGGA	45.0	45.0	57.9	58.1	2161-2172 bp	0.25	50.5
rpl16_p5	TCAGCAATAGTGTCCTTGCC	AAATTGCAGGGCGTTTAGGA	50.0	45.0	57.9	58.1	2387-2405 bp	0.25	50.0