Hotspot

rpl32-trnL-UAG

SSC · 105949-106423 · 475 bp

Back to Aristida

Pi 0.0312

MarkerSeek score 72.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 475
variable_sites: 47
pi: 0.0312
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGTTTTTCTGGGCAACAAACA	TCCTAAGAGCAGCGTGTCTA	42.9	50.0	58.0	57.9	564-581 bp	1.0	78.3
rpl32-trnL-UAG_p2	GGTTTTTCTGGGCAACAAACA	AGCAGCGTGTCTACCAATTT	42.9	45.0	58.0	57.8	557-574 bp	1.0	77.9
rpl32-trnL-UAG_p3	GGTTTTTCTGGGCAACAAACA	CCTAAGAGCAGCGTGTCTAC	42.9	55.0	58.0	57.8	563-580 bp	1.0	77.7
rpl32-trnL-UAG_p4	GGTTTTTCTGGGCAACAAACA	CTAAGAGCAGCGTGTCTACC	42.9	55.0	58.0	57.8	562-579 bp	1.0	77.7
rpl32-trnL-UAG_p5	GGTTTTTCTGGGCAACAAACA	GCTTGAGCACTGCTTCCTAA	42.9	50.0	58.0	58.2	578-595 bp	1.0	77.6