Hotspot

ndhF-rpl32

SSC · 105005-105747 · 743 bp

Back to Aristida

Pi 0.0204

MarkerSeek score 66.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 743
variable_sites: 44
pi: 0.0204
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGATTACCCAGGCATATTGA	TGACATCGAAGTACGTTTTT	45.0	35.0	54.5	53.2	795-838 bp	1.0	46.4
ndhF-rpl32_p2	GGATTACCCAGGCATATTGA	TTGACATCGAAGTACGTTTT	45.0	35.0	54.5	53.2	796-839 bp	1.0	46.4
ndhF-rpl32_p3	GGATTACCCAGGCATATTGA	TTTGACATCGAAGTACGTTT	45.0	35.0	54.5	53.2	797-840 bp	1.0	46.4
ndhF-rpl32_p4	GGATTACCCAGGCATATTGA	TTTTGACATCGAAGTACGTT	45.0	35.0	54.5	53.2	798-841 bp	1.0	46.4
ndhF-rpl32_p5	GGATTACCCAGGCATATTGA	TCCAAAGATTTTTACGAATACGCT	45.0	33.3	54.5	57.3	823-866 bp	1.0	46.2