Hotspot

rpl14-rpl16

LSC · 77172-77284 · 113 bp

Back to Aristida

Pi 0.0344
MarkerSeek score 65.20
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
113
variable_sites
8
pi
0.0344
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.96
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 GCTCCACTGTTATCCGCTAC TAGCTGCCAGTAAAATGCCC 55.0 50.0 58.1 58.2 208-213 bp 1.0 82.6
rpl14-rpl16_p2 AGCTCCACTGTTATCCGCTA TAGCTGCCAGTAAAATGCCC 50.0 50.0 58.2 58.2 209-214 bp 1.0 82.0
rpl14-rpl16_p3 GCTCCACTGTTATCCGCTAC GCCAGTAAAATGCCCATACG 55.0 50.0 58.1 57.2 203-208 bp 1.0 78.5
rpl14-rpl16_p4 AGCTCCACTGTTATCCGCTA GCCAGTAAAATGCCCATACG 50.0 50.0 58.2 57.2 204-209 bp 1.0 77.8
rpl14-rpl16_p5 GCTCCACTGTTATCCGCTAC GCTGCCAGTAAAATGCCCAT 55.0 50.0 58.1 59.2 206-211 bp 1.0 75.2