Hotspot

psaJ-rpl33

LSC · 64288-64788 · 501 bp

Back to Aristida

Pi 0.0211

MarkerSeek score 65.00

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 501
variable_sites: 32
pi: 0.0211
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GGTTTGGAGCTTTAGCAGGT	TCTAACATCTTTGCCCTTGG	50.0	45.0	58.1	55.3	602-650 bp	1.0	62.5
psaJ-rpl33_p2	TGGTTTGGAGCTTTAGCAGG	TCTAACATCTTTGCCCTTGG	50.0	45.0	58.1	55.3	603-651 bp	1.0	62.5
psaJ-rpl33_p3	CAGATGCTTTGTCGTTCCCT	TCTAACATCTTTGCCCTTGG	50.0	45.0	58.2	55.3	554-602 bp	1.0	62.1
psaJ-rpl33_p4	TCCCAGATGCTTTGTCGTTC	TCTAACATCTTTGCCCTTGG	50.0	45.0	58.2	55.3	557-605 bp	1.0	62.1
psaJ-rpl33_p5	TTCCCAGATGCTTTGTCGTT	TCTAACATCTTTGCCCTTGG	45.0	45.0	57.7	55.3	558-606 bp	1.0	61.4