Hotspot

petA-psbJ

LSC · 60032-60970 · 939 bp

Back to Aristida

Pi 0.0173

MarkerSeek score 61.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 939
variable_sites: 49
pi: 0.0173
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	TCTGGATTGGGTTCATCTCT	50.0	45.0	57.9	55.5	1053-1096 bp	1.0	64.3
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	TTCTGGATTGGGTTCATCTCT	50.0	42.9	57.9	56.3	1054-1097 bp	1.0	62.8
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTCT	TCTGGATTGGGTTCATCTCT	50.0	45.0	58.7	55.5	1060-1103 bp	1.0	59.3
petA-psbJ_p4	GGCCTTTTGTTCTTCTTCGC	ATTCTGGATTGGGTTCATCTCT	50.0	40.9	57.9	56.9	1055-1098 bp	1.0	59.0
petA-psbJ_p5	GCCTTTTGTTCTTCTTCGCA	TCTGGATTGGGTTCATCTCT	45.0	45.0	57.2	55.5	1052-1095 bp	1.0	58.6