Hotspot

trnC-GCA-rpoB

LSC · 18713-19889 · 1177 bp

Back to Aristida

Pi 0.0165

MarkerSeek score 63.00

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1177
variable_sites: 57
pi: 0.0165
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	ATCCAGTATCGCCGGAACTA	AGTTCTTCTGCCAAACCTCG	50.0	50.0	58.0	58.1	1409-1484 bp	0.75	75.8
trnC-GCA-rpoB_p2	ATCCAGTATCGCCGGAACTA	AATTCCGATCTTCCTCCCCA	50.0	50.0	58.0	58.1	1778-1853 bp	0.75	74.7
trnC-GCA-rpoB_p3	ATCCAGTATCGCCGGAACTA	ATTCCGATCTTCCTCCCCAA	50.0	50.0	58.0	58.1	1777-1852 bp	0.75	74.7
trnC-GCA-rpoB_p4	ATCCAGTATCGCCGGAACTA	TTCCGATCTTCCTCCCCAAT	50.0	50.0	58.0	58.1	1776-1851 bp	0.75	74.7
trnC-GCA-rpoB_p5	ATCCAGTATCGCCGGAACTA	CCCCACCTACACAAGCAAAT	50.0	50.0	58.0	58.1	2005-2080 bp	0.375	57.8