Hotspot

petN-trnC-GCA

LSC · 17689-18641 · 953 bp

Back to Aristida

Pi 0.0173

MarkerSeek score 69.80

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 953
variable_sites: 50
pi: 0.0173
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	50.0	52.6	58.2	58.1	847-1002 bp	1.0	78.5
petN-trnC-GCA_p2	AGCCCAAGCGAGACTTACTA	CCAGTTCAAATCTGGGTGCC	50.0	55.0	58.2	59.1	849-1004 bp	1.0	78.1
petN-trnC-GCA_p3	CCACTCCTCCCCCATACTAC	AGTTCAAATCTGGGTGCCG	60.0	52.6	58.3	58.1	903-1058 bp	1.0	77.3
petN-trnC-GCA_p4	CCACTCCTCCCCCATACTAC	CCAGTTCAAATCTGGGTGCC	60.0	55.0	58.3	59.1	905-1060 bp	1.0	76.8
petN-trnC-GCA_p5	TCCACTCCTCCCCCATACTA	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.4	58.1	904-1059 bp	1.0	76.5