Hotspot

trnD-GUC-psbM

LSC · 15997-17042 · 1046 bp

Back to Aristida

Pi 0.0172
MarkerSeek score 62.90
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
LSC
length_bp
1046
variable_sites
57
pi
0.0172
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 CGCTATCGTCTAGTGATGCC TAGTAAGTCTCGCTTGGGCT 55.0 50.0 58.0 58.2 2324-2361 bp 1.0 84.7
trnD-GUC-psbM_p2 GGCGCTATCGTCTAGTGATG TAGTAAGTCTCGCTTGGGCT 55.0 50.0 58.0 58.2 2326-2363 bp 1.0 84.7
trnD-GUC-psbM_p3 AATTCTTCCTGGGTCGATGC TAGGGCCGAATCACCTTTTG 50.0 50.0 58.0 57.9 2289-2328 bp 0.875 79.5
trnD-GUC-psbM_p4 CGCTATCGTCTAGTGATGCC TAGGGCCGAATCACCTTTTG 55.0 50.0 58.0 57.9 2430-2461 bp 0.875 79.3
trnD-GUC-psbM_p5 GGCGCTATCGTCTAGTGATG TAGGGCCGAATCACCTTTTG 55.0 50.0 58.0 57.9 2432-2463 bp 0.875 79.3