Hotspot

trnS-GCU-psbD

LSC · 8344-9355 · 1012 bp

Back to Aristida

Pi 0.0108
MarkerSeek score 67.20
Primer available yes
Label trnS-GCU-psbD

Feature Metadata

label_name
trnS-GCU-psbD
feature_type
igs
region
LSC
length_bp
1012
variable_sites
34
pi
0.0108
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-psbD_p1 ATTAGCAATCCGCCGCTTTA GCCGGACCATCCTACAAAAA 45.0 50.0 58.0 58.2 1134-1158 bp 1.0 84.4
trnS-GCU-psbD_p2 ATTAGCAATCCGCCGCTTTA ACAAAAACGAAACGGTCCCT 45.0 45.0 58.0 58.2 1121-1145 bp 1.0 83.8
trnS-GCU-psbD_p3 GCTTTAGTCCACTCAGCCAT GCCGGACCATCCTACAAAAA 50.0 50.0 57.6 58.2 1120-1144 bp 1.0 81.5
trnS-GCU-psbD_p4 ATTAGCAATCCGCCGCTTTA GAAACGGTCCCTTCGTAACC 45.0 55.0 58.0 58.6 1113-1137 bp 1.0 81.3
trnS-GCU-psbD_p5 GCTTTAGTCCACTCAGCCAT ACAAAAACGAAACGGTCCCT 50.0 45.0 57.6 58.2 1107-1131 bp 1.0 81.0