Hotspot

rps16-trnQ-UUG

LSC · 5558-7035 · 1478 bp

Back to Aristida

Pi 0.0185
MarkerSeek score 59.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1478
variable_sites
60
pi
0.0185
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.70
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 566-1610 bp 1.0 86.5
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 577-1621 bp 1.0 85.9
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 570-1614 bp 1.0 85.4
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.2 57.8 581-1625 bp 1.0 84.8
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA GCATAAAACCAAATGGGGCG 45.0 50.0 58.1 58.3 627-1671 bp 1.0 84.1