Hotspot

atpF

LSC · 15032-16900 · 1869 bp

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Pi 0.0623

MarkerSeek score 84.40

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1869
variable_sites: 318
pi: 0.0623
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CCTACTTGAAGTACGGTGCC	CCTCTCGCTTTCTTCCTTCC	55.0	55.0	58.0	58.0	1903-2992 bp	1.0	91.8
atpF_p2	CCTACTTGAAGTACGGTGCC	CTCTCGCTTTCTTCCTTCCC	55.0	55.0	58.0	58.0	1902-2991 bp	1.0	91.8
atpF_p3	CCTACTTGAAGTACGGTGCC	ATTTATGGACTGGTCGTGGC	55.0	50.0	58.0	58.0	2114-2850 bp	0.923077	88.6
atpF_p4	CCTACTTGAAGTACGGTGCC	CGGGAAGGACGGATTTTAGG	55.0	55.0	58.0	58.1	1940-2675 bp	0.923077	88.5
atpF_p5	ATACGAGCAATTCCGTCACC	CTCTCGCTTTCTTCCTTCCC	50.0	55.0	58.1	58.0	1920-2655 bp	0.923077	88.3