Hotspot

ycf1-trnN-GUU

IRa · 144179-146194 · 2016 bp

Back to Arisaema

Pi 0.0425

MarkerSeek score 76.00

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 2016
variable_sites: 267
pi: 0.0425
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	TTCCCCCGTTACTGAGGTTT	AGGATTCGAACCTACGACCA	50.0	50.0	58.6	58.2	1263-2776 bp	0.923077	83.2
ycf1-trnN-GUU_p2	TTCCCCCGTTACTGAGGTTT	AATCAGTTAACAGCCGACCG	50.0	50.0	58.6	58.3	1244-2757 bp	0.923077	82.2
ycf1-trnN-GUU_p3	TTCCCCCGTTACTGAGGTTT	CCCCAAGTAGGATTCGAACC	50.0	55.0	58.6	57.7	1271-2784 bp	0.923077	81.8
ycf1-trnN-GUU_p4	CCCGTTACTGAGGTTTCCTT	AGGATTCGAACCTACGACCA	50.0	50.0	57.1	58.2	1259-2772 bp	0.923077	80.8
ycf1-trnN-GUU_p5	CCCGTTACTGAGGTTTCCTT	AATCAGTTAACAGCCGACCG	50.0	50.0	57.1	58.3	1240-2753 bp	0.923077	79.9