Hotspot

rps15-ycf1

SSC · 137920-139144 · 1225 bp

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Pi 0.113

MarkerSeek score 75.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 1225
variable_sites: 412
pi: 0.113
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCCCAACGCGTTACATAAGG	TGGAAGGGGAATGCTGTAGA	50.0	50.0	58.0	58.0	1388-2919 bp	1.0	91.6
rps15-ycf1_p2	TCCCAACGCGTTACATAAGG	GAAGGGGAATGCTGTAGACG	50.0	55.0	58.0	58.1	1386-2917 bp	1.0	91.4
rps15-ycf1_p3	AACACCATGCATTGATGGGT	TGGAAGGGGAATGCTGTAGA	45.0	50.0	58.1	58.0	1251-2782 bp	1.0	91.3
rps15-ycf1_p4	TTCCCAATTGTTCTGGTGCA	TGGAAGGGGAATGCTGTAGA	45.0	50.0	57.9	58.0	1447-2978 bp	1.0	91.1
rps15-ycf1_p5	TCCCAACGCGTTACATAAGG	GCGCCTAGACGTAATGTTGA	50.0	50.0	58.0	58.1	1731-2848 bp	0.923077	88.2