Hotspot

psaC-ndhE

SSC · 130170-131012 · 843 bp

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Pi 0.0475

MarkerSeek score 82.10

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 843
variable_sites: 150
pi: 0.0475
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	AGCTTTACATCCCTGCCAAG	CGTAACAGAAAATCAACGCGT	50.0	42.9	57.9	57.8	956-1379 bp	1.0	81.6
psaC-ndhE_p2	GGCCCGTACACATTGAGTAC	CGTAACAGAAAATCAACGCGT	55.0	42.9	58.4	57.8	908-1331 bp	1.0	79.9
psaC-ndhE_p3	GCCCGTACACATTGAGTACA	CGTAACAGAAAATCAACGCGT	50.0	42.9	57.6	57.8	907-1330 bp	1.0	79.7
psaC-ndhE_p4	AGCTTTACATCCCTGCCAAG	TCGTAACAGAAAATCAACGCG	50.0	42.9	57.9	57.6	957-1380 bp	1.0	79.5
psaC-ndhE_p5	ACATCCCTGCCAAGGTATCA	CGTAACAGAAAATCAACGCGT	50.0	42.9	58.4	57.8	950-1373 bp	1.0	79.5