Hotspot

trnN-GUU-ndhF

IRb · 121214-122365 · 1152 bp

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Pi 0.0903

MarkerSeek score 78.70

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1152
variable_sites: 353
pi: 0.0903
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	CGGGAGAATTGATGACTGCA	ACACAAGGGTTGTAATCCGG	50.0	50.0	58.0	57.8	1377-2351 bp	1.0	90.2
trnN-GUU-ndhF_p2	CGGGAGAATTGATGACTGCA	TGCTTTTGGTCAAGGGGAAA	50.0	45.0	58.0	57.8	1691-2665 bp	0.923077	87.4
trnN-GUU-ndhF_p3	CGGGAGAATTGATGACTGCA	TTGCTTTTGGTCAAGGGGAA	50.0	45.0	58.0	57.8	1692-2666 bp	0.923077	87.4
trnN-GUU-ndhF_p4	GCCAGGTATTGACGGTGATT	TGCTTTTGGTCAAGGGGAAA	50.0	45.0	58.0	57.8	1576-2550 bp	0.923077	87.3
trnN-GUU-ndhF_p5	GCCAGGTATTGACGGTGATT	TTGCTTTTGGTCAAGGGGAA	50.0	45.0	58.0	57.8	1577-2551 bp	0.923077	87.3