Hotspot

rpl16-rps3

LSC · 93169-94454 · 1286 bp

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Pi 0.0309

MarkerSeek score 76.20

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1286
variable_sites: 143
pi: 0.0309
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	CAAGTGCCTGAAGAGCGTAT	TGGATATTTGTAGACGGAAAA	50.0	33.3	58.0	52.6	1305-1697 bp	1.0	52.0
rpl16-rps3_p2	CAAGTGCCTGAAGAGCGTAT	TTGGATATTTGTAGACGGAAA	50.0	33.3	58.0	52.6	1306-1698 bp	1.0	52.0
rpl16-rps3_p3	CAAGTGCCTGAAGAGCGTAT	TTTGGATATTTGTAGACGGAA	50.0	33.3	58.0	52.6	1307-1699 bp	1.0	52.0
rpl16-rps3_p4	CAAGTGCCTGAAGAGCGTAT	TTGGATATTTGTAGACGGAAAA	50.0	31.8	58.0	53.5	1306-1698 bp	1.0	52.0
rpl16-rps3_p5	CAAGTGCCTGAAGAGCGTAT	TTTGGATATTTGTAGACGGAAA	50.0	31.8	58.0	53.5	1307-1699 bp	1.0	52.0