Hotspot

rpl33-rps18

LSC · 78640-79213 · 574 bp

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Pi 0.105

MarkerSeek score 84.00

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 574
variable_sites: 154
pi: 0.105
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	GACACAATACACCCAGTCGA	ATTCGATCCCCCGATCCAAT	50.0	50.0	57.3	58.6	353-750 bp	1.0	81.0
rpl33-rps18_p2	CGACACAATACACCCAGTCG	ATTCGATCCCCCGATCCAAT	55.0	50.0	58.7	58.6	354-744 bp	0.923077	78.6
rpl33-rps18_p3	TCGACACAATACACCCAGTC	ATTCGATCCCCCGATCCAAT	50.0	50.0	57.3	58.6	355-745 bp	0.923077	77.9
rpl33-rps18_p4	CGACACAATACACCCAGTCG	ATCAATTCGATCCCCCGATC	55.0	50.0	58.7	57.2	358-748 bp	0.923077	77.5
rpl33-rps18_p5	AGCATACAATTCACGGGGAA	ATTCGATCCCCCGATCCAAT	45.0	50.0	57.2	58.6	299-696 bp	0.923077	77.4