Hotspot

psaJ-rpl33

LSC · 77989-78438 · 450 bp

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Pi 0.0203

MarkerSeek score 75.60

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 450
variable_sites: 36
pi: 0.0203
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATCGTTTATTCCCGGATGCC	CGTTTCGGACACAACTGGTA	50.0	50.0	58.1	58.1	510-561 bp	1.0	88.1
psaJ-rpl33_p2	CGGATGCCTTGTCATTTCCT	CGTTTCGGACACAACTGGTA	50.0	50.0	58.2	58.1	498-549 bp	1.0	87.1
psaJ-rpl33_p3	TATCTCTCTGTGGCACCTGT	CGTTTCGGACACAACTGGTA	50.0	50.0	57.8	58.1	580-631 bp	1.0	86.5
psaJ-rpl33_p4	TGGTTTGGGTCTTTAGCAGG	CGTTTCGGACACAACTGGTA	50.0	50.0	57.7	58.1	547-598 bp	1.0	86.4
psaJ-rpl33_p5	GGTTTGGGTCTTTAGCAGGT	CGTTTCGGACACAACTGGTA	50.0	50.0	57.7	58.1	546-597 bp	1.0	86.4