Hotspot

rpoB-trnC-GCA

LSC · 30855-32626 · 1772 bp

Back to Arisaema

Pi 0.0691

MarkerSeek score 85.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1772
variable_sites: 363
pi: 0.0691
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCCTCATTTTCATCCCGGAG	GCGACACCCAGATTTGAACT	55.0	50.0	58.0	58.5	1485-2110 bp	1.0	88.0
rpoB-trnC-GCA_p2	CTCATTTTCATCCCGGAGCA	GCGACACCCAGATTTGAACT	50.0	50.0	58.0	58.5	1483-2108 bp	1.0	87.8
rpoB-trnC-GCA_p3	CCCTCATTTTCATCCCGGAG	TAAAGGATTTGCAGTCCCCC	55.0	50.0	58.0	57.5	1460-2085 bp	1.0	87.6
rpoB-trnC-GCA_p4	CCCTCATTTTCATCCCGGAG	CCTTACCACTTGGCCATGTC	55.0	55.0	58.0	58.5	1439-2064 bp	1.0	87.5
rpoB-trnC-GCA_p5	CTCATTTTCATCCCGGAGCA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.0	57.5	1458-2083 bp	1.0	87.5