Hotspot

trnS-GCU-trnS-CGA

LSC · 9769-12266 · 2498 bp

Back to Arisaema

Pi 0.108

MarkerSeek score 75.50

Primer available yes

Label trnS-GCU-trnS-CGA

Feature Metadata

label_name: trnS-GCU-trnS-CGA
feature_type: igs
region: LSC
length_bp: 2498
variable_sites: 650
pi: 0.108
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-CGA_p1	GAGAAAGCGGAGAAAGAGGG	TACCACTAAACTATACCCGC	55.0	45.0	58.0	54.0	1486-2613 bp	1.0	59.8
trnS-GCU-trnS-CGA_p2	ATTAGCAATCCGCCGCTTTA	TACCACTAAACTATACCCGC	45.0	45.0	58.0	54.0	1431-2558 bp	1.0	59.6
trnS-GCU-trnS-CGA_p3	GAGAAAGCGGAGAAAGAGGG	TTACCACTAAACTATACCCGC	55.0	42.9	58.0	54.9	1487-2614 bp	1.0	58.7
trnS-GCU-trnS-CGA_p4	ATTAGCAATCCGCCGCTTTA	TTACCACTAAACTATACCCGC	45.0	42.9	58.0	54.9	1432-2559 bp	1.0	58.5
trnS-GCU-trnS-CGA_p5	AGAAAGCGGAGAAAGAGGGA	TACCACTAAACTATACCCGC	50.0	45.0	58.4	54.0	1485-2612 bp	1.0	57.1