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trnH-GUG-psbA

LSC · 59-844 · 786 bp

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Pi 0.115

MarkerSeek score 81.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 786
variable_sites: 245
pi: 0.115
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CAATCCACTGCCTTGATCCA	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	696-1168 bp	1.0	89.6
trnH-GUG-psbA_p2	AATCCACTGCCTTGATCCAC	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	695-1167 bp	1.0	89.5
trnH-GUG-psbA_p3	ACAATCCACTGCCTTGATCC	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	697-1169 bp	1.0	89.5
trnH-GUG-psbA_p4	CAATCCACTGCCTTGATCCA	ATCAACCGTGCTAACCTTGG	50.0	50.0	57.9	58.2	698-1170 bp	1.0	89.5
trnH-GUG-psbA_p5	AATCCACTGCCTTGATCCAC	ATCAACCGTGCTAACCTTGG	50.0	50.0	57.9	58.2	697-1169 bp	1.0	89.5