Hotspot

rps16

LSC · 5252-6385 · 1134 bp

Pi 0.00103

MarkerSeek score 57.10

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1134
variable_sites: 2
pi: 0.00103
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGAAACAAAAAGGTTATACAAGGCT	TATGTACCGACCGAACCGAT	32.0	50.0	57.4	58.3	1270-1272 bp	1.0	40.2
rps16_p2	AGAAACAAAAAGGTTATACAAGGCT	CCGACCGAACCGATGATTAT	32.0	50.0	57.4	57.6	1264-1266 bp	1.0	40.2
rps16_p3	ACAAAAAGGTTATACAAGGCT	TATGTACCGACCGAACCGAT	33.3	50.0	53.3	58.3	1266-1268 bp	1.0	40.2
rps16_p4	AGAAACAAAAAGGTTATACAAGGCT	ATTATGTACCGACCGAACCG	32.0	50.0	57.4	57.5	1272-1274 bp	1.0	40.2
rps16_p5	GGAAATAGCAAAGAAACAAAAAGGT	TATGTACCGACCGAACCGAT	32.0	50.0	57.2	58.3	1281-1283 bp	1.0	40.2