Hotspot

ndhG-ndhI

SSC · 124060-124469 · 410 bp

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Pi 0.00569
MarkerSeek score 57.70
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
410
variable_sites
4
pi
0.00569
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 AATCATGTATTGGTCCGGGC TCAAATTGCTTTGGGTCGTT 50.0 40.0 58.0 56.4 503-526 bp 1.0 71.5
ndhG-ndhI_p2 AATACCACCCCCATACCTCC TCAAATTGCTTTGGGTCGTT 55.0 40.0 58.2 56.4 558-581 bp 1.0 69.9
ndhG-ndhI_p3 TGTATTGGTCCGGGCAAATC TCAAATTGCTTTGGGTCGTT 50.0 40.0 58.2 56.4 498-521 bp 1.0 69.8
ndhG-ndhI_p4 AATCATGTATTGGTCCGGGC ATCAAATTGCTTTGGGTCGT 50.0 40.0 58.0 56.2 504-527 bp 1.0 69.7
ndhG-ndhI_p5 AATCATGTATTGGTCCGGGC TCAAATTGCTTTGGGTCGTTT 50.0 38.1 58.0 57.2 503-526 bp 1.0 69.5