Hotspot

ndhF-rpl32

SSC · 116783-117861 · 1079 bp

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Pi 0.00247
MarkerSeek score 61.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1079
variable_sites
5
pi
0.00247
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTGTTGCCGTTGGAAAAAGT AAAAGCTTTCAATGCTGCCC 40.0 45.0 57.0 57.8 1273-1277 bp 1.0 72.0
ndhF-rpl32_p2 TTGTTGCCGTTGGAAAAAGT AAGCTTTCAATGCTGCCCTA 40.0 45.0 57.0 57.8 1271-1275 bp 1.0 71.6
ndhF-rpl32_p3 TTGTTGCCGTTGGAAAAAGT GCTGCCCTATATCCCTTCCT 40.0 55.0 57.0 58.3 1260-1264 bp 1.0 70.7
ndhF-rpl32_p4 TTGTTGCCGTTGGAAAAAGT AGCTTTCAATGCTGCCCTAT 40.0 45.0 57.0 57.6 1270-1274 bp 1.0 69.8
ndhF-rpl32_p5 TTTGTTGCCGTTGGAAAAAGT AAAAGCTTTCAATGCTGCCC 38.1 45.0 57.7 57.8 1274-1278 bp 1.0 69.7