Hotspot

accD-psaI

LSC · 62167-62969 · 803 bp

Back to Aria

Pi 0.00353
MarkerSeek score 59.10
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
803
variable_sites
4
pi
0.00353
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 AGGGCGTTCTGAGTGAGTTA CCATTGCAATTGCCGGAAAT 50.0 45.0 58.4 58.0 927-1068 bp 1.0 79.4
accD-psaI_p2 AGGGCGTTCTGAGTGAGTTA AATGGAGGGGAGACTGTTGA 50.0 50.0 58.4 58.0 884-1025 bp 1.0 79.4
accD-psaI_p3 AGGGCGTTCTGAGTGAGTTA TTGCCGGAAATACTAAGCCC 50.0 50.0 58.4 58.0 918-1059 bp 1.0 79.2
accD-psaI_p4 AGGGCGTTCTGAGTGAGTTA GAGGGGAGACTGTTGAGAGT 50.0 55.0 58.4 58.1 880-1021 bp 1.0 79.2
accD-psaI_p5 AGGGCGTTCTGAGTGAGTTA GCACAAAAATGGAGGGGAGA 50.0 50.0 58.4 58.1 891-1032 bp 1.0 79.1