Hotspot

ndhC-trnV-UAC

LSC · 54237-54798 · 562 bp

Back to Aria

Pi 0.00919
MarkerSeek score 57.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
562
variable_sites
10
pi
0.00919
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCGGAAAT GCACGTTGGGTCTTTGAAAC 50.0 50.0 57.9 58.2 757-918 bp 1.0 78.8
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 693-854 bp 1.0 78.6
ndhC-trnV-UAC_p3 GGGGCTAAAACTCCGGAAAT AATGCACGTTGGGTCTTTGA 50.0 45.0 57.9 58.3 760-921 bp 1.0 77.6
ndhC-trnV-UAC_p4 GGGGCTAAAACTCCGGAAAT ATGCACGTTGGGTCTTTGAA 50.0 45.0 57.9 58.3 759-920 bp 1.0 77.6
ndhC-trnV-UAC_p5 GGGGCTAAAACTCCGGAAAT AAATGCACGTTGGGTCTTTG 50.0 45.0 57.9 57.5 761-922 bp 1.0 76.0