Hotspot

trnF-GAA-ndhJ

LSC · 51833-52531 · 699 bp

Back to Aria

Pi 0.000715
MarkerSeek score 60.40
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
699
variable_sites
1
pi
0.000715
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA ATCCACGCCTGAAACGTATC 55.0 50.0 58.0 58.1 868-874 bp 1.0 80.2
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA ATCCACGCCTGAAACGTATC 50.0 50.0 58.1 58.1 832-838 bp 1.0 79.8
trnF-GAA-ndhJ_p3 GTCGTGAATGGTCGGGATAG ATCCACGCCTGAAACGTATC 55.0 50.0 57.9 58.1 881-887 bp 1.0 79.1
trnF-GAA-ndhJ_p4 CTGGTAGAGCAGAGGACTGA ATCCACGCCTGAAACGTATC 55.0 50.0 57.9 58.1 856-862 bp 1.0 79.1
trnF-GAA-ndhJ_p5 GAGGATGATGCGTCGTGAAT ATCCACGCCTGAAACGTATC 50.0 50.0 58.1 58.1 892-898 bp 1.0 79.1