Hotspot

rps4-trnT-UGU

LSC · 49008-49426 · 419 bp

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Pi 0.0203
MarkerSeek score 48.20
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
419
variable_sites
17
pi
0.0203
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 TAAGATCGCTTCCGGCTCTA CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.0 57.9 578-608 bp 1.0 81.1
rps4-trnT-UGU_p2 TTTCTAAGATCGCTTCCGGC CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.1 57.9 582-612 bp 1.0 80.7
rps4-trnT-UGU_p3 CTTCCGGCTCTAGGCTTTTT CTCAGAGGTTAGAGCATCGC 50.0 55.0 57.9 57.9 570-600 bp 1.0 80.3
rps4-trnT-UGU_p4 TAAGATCGCTTCCGGCTCTA ATGGTCATCGGTTCGATTCC 50.0 50.0 58.0 57.8 546-576 bp 1.0 80.3
rps4-trnT-UGU_p5 TTTCTAAGATCGCTTCCGGC ATGGTCATCGGTTCGATTCC 50.0 50.0 58.1 57.8 550-580 bp 1.0 80.0