Hotspot

trnC-GCA-petN

LSC · 29204-30120 · 917 bp

Back to Aria

Pi 0.00182
MarkerSeek score 60.50
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
917
variable_sites
3
pi
0.00182
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG CAGCCCAAGCGAGATTTACT 50.0 50.0 58.1 58.0 980-1010 bp 1.0 79.7
trnC-GCA-petN_p2 GTATCATTTTGGCGGCATGG CAGCCCAAGCGAGATTTACT 50.0 50.0 57.9 58.0 1032-1062 bp 1.0 79.2
trnC-GCA-petN_p3 TTTCCCCAGTTCAAATCCGG AGTCCACTCCTTCCCCATAC 50.0 55.0 58.1 58.1 1038-1068 bp 1.0 78.9
trnC-GCA-petN_p4 TTTCCCCAGTTCAAATCCGG GTCCACTCCTTCCCCATACT 50.0 55.0 58.1 58.1 1037-1067 bp 1.0 78.9
trnC-GCA-petN_p5 GTATCATTTTGGCGGCATGG GTCCACTCCTTCCCCATACT 50.0 55.0 57.9 58.1 1089-1119 bp 1.0 78.4