Hotspot

atpH-atpI

LSC · 14470-15613 · 1144 bp

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Pi 0.00102
MarkerSeek score 59.70
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1144
variable_sites
2
pi
0.00102
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 AATAGAAGCAAGCCCGACAG GCGAATCCATGGAAGGTCAT 50.0 50.0 58.0 58.0 1233-1245 bp 1.0 79.9
atpH-atpI_p2 ATAACGGAAGCGGCAGAAAT GCGAATCCATGGAAGGTCAT 45.0 50.0 58.0 58.0 1199-1211 bp 1.0 79.8
atpH-atpI_p3 AATAGAAGCAAGCCCGACAG TTTTTGCAACCTTAGCTGCG 50.0 45.0 58.0 57.9 1263-1275 bp 1.0 79.0
atpH-atpI_p4 ATAACGGAAGCGGCAGAAAT TTTTTGCAACCTTAGCTGCG 45.0 45.0 58.0 57.9 1229-1241 bp 1.0 79.0
atpH-atpI_p5 AATAACGGAAGCGGCAGAAA GCGAATCCATGGAAGGTCAT 45.0 50.0 58.2 58.0 1200-1212 bp 1.0 78.6