Hotspot

trnR-UCU-atpA

LSC · 10143-10882 · 740 bp

Back to Aria

Pi 0.0171
MarkerSeek score 75.70
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
740
variable_sites
25
pi
0.0171
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 TGGAATGAAAAGCGTCCATTG AGACATTTACCGACGAAGCG 42.9 50.0 57.4 58.4 909-953 bp 1.0 69.9
trnR-UCU-atpA_p2 GGAATGAAAAGCGTCCATTGT AGACATTTACCGACGAAGCG 42.9 50.0 57.4 58.4 908-952 bp 1.0 69.8
trnR-UCU-atpA_p3 GGTTCAAATCCTATTGGACGC AGACATTTACCGACGAAGCG 47.6 50.0 57.3 58.4 848-892 bp 1.0 69.3
trnR-UCU-atpA_p4 TGGATAGGACAGAGGTCTTCT AGACATTTACCGACGAAGCG 47.6 50.0 56.9 58.4 883-927 bp 1.0 66.2
trnR-UCU-atpA_p5 TGGAATGAAAAGCGTCCATT AGACATTTACCGACGAAGCG 40.0 50.0 55.9 58.4 909-953 bp 1.0 65.8