Hotspot

trnH-GUG-psbA

LSC · 142-410 · 269 bp

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Pi 0.0149
MarkerSeek score 59.70
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
269
variable_sites
7
pi
0.0149
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 ATATTATGGGCGAACGACGG CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 390-405 bp 1.0 83.1
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 379-394 bp 1.0 83.1
trnH-GUG-psbA_p3 ATATTATGGGCGAACGACGG TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 393-408 bp 1.0 83.0
trnH-GUG-psbA_p4 ATATTATGGGCGAACGACGG GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 440-455 bp 1.0 82.9
trnH-GUG-psbA_p5 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 393-408 bp 1.0 82.9