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psbZ-trnG-GCC

LSC · 36665-37017 · 353 bp

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Pi 0.0437
MarkerSeek score 73.40
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
353
variable_sites
50
pi
0.0437
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.94
markerseek_score
73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TTTGCTTCTCCTGAGGGTTG TACCATTCGACCATATCCGC 50.0 50.0 57.7 57.3 426-504 bp 0.933333 81.2
psbZ-trnG-GCC_p2 GGGTTGGTCGGGTAACAAAA TACCATTCGACCATATCCGC 50.0 50.0 58.3 57.3 412-490 bp 0.933333 81.0
psbZ-trnG-GCC_p3 TTTGCTTCTCCTGAGGGTTG TTACCATTCGACCATATCCGC 50.0 47.6 57.7 58.0 427-505 bp 0.933333 78.8
psbZ-trnG-GCC_p4 GGGTTGGTCGGGTAACAAAA TTACCATTCGACCATATCCGC 50.0 47.6 58.3 58.0 413-491 bp 0.933333 78.6
psbZ-trnG-GCC_p5 TTTGCTTCTCCTGAGGGTTG ACCATTCGACCATATCCGC 50.0 52.6 57.7 57.4 425-503 bp 0.933333 74.3