Hotspot

trnT-GGU-psbD

LSC · 32080-33311 · 1232 bp

Back to Argentina

Pi 0.0279

MarkerSeek score 80.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1232
variable_sites: 137
pi: 0.0279
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	TCCCTCCGTAACCAGTCATC	55.0	55.0	58.1	58.5	1354-1511 bp	1.0	86.9
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	TCCCTCCGTAACCAGTCATC	50.0	55.0	58.1	58.5	1357-1514 bp	1.0	86.7
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	TCCCTCCGTAACCAGTCATC	50.0	55.0	58.3	58.5	1331-1488 bp	1.0	85.3
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	GTCCCTCCGTAACCAGTCAT	55.0	55.0	58.1	58.8	1355-1512 bp	1.0	84.6
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CCTCCGTAACCAGTCATCCA	55.0	55.0	58.1	58.8	1352-1509 bp	1.0	84.6