Hotspot

petN-psbM

LSC · 28880-29814 · 935 bp

Back to Argentina

Pi 0.0388

MarkerSeek score 81.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 935
variable_sites: 154
pi: 0.0388
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATGGGGAAGAAGTGGACTCT	AAAACTGTCAGCCAAGGTGA	50.0	45.0	57.6	57.6	688-1347 bp	1.0	85.7
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	AAACTGTCAGCCAAGGTGAT	50.0	45.0	57.6	57.3	687-1346 bp	1.0	83.9
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	AACTGTCAGCCAAGGTGATT	50.0	45.0	57.6	57.3	686-1345 bp	1.0	83.9
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	AAAACTGTCAGCCAAGGTGAT	50.0	42.9	57.6	58.1	688-1347 bp	1.0	80.8
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	AAACTGTCAGCCAAGGTGATT	50.0	42.9	57.6	58.1	687-1346 bp	1.0	80.8