Hotspot

trnR-UCU-atpA

LSC · 10092-10634 · 543 bp

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Pi 0.0601

MarkerSeek score 83.80

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 543
variable_sites: 120
pi: 0.0601
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	TGGAACGTTTTCTACTTCAGGA	47.6	40.9	57.3	57.3	597-740 bp	1.0	57.0
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	TGGAACGTTTTCTACTTCAGGAA	47.6	39.1	57.3	58.0	597-740 bp	1.0	54.2
trnR-UCU-atpA_p3	TGGATAGGACAGAGGTCTTCT	TGGAACGTTTTCTACTTCAGGA	47.6	40.9	56.9	57.3	632-775 bp	1.0	54.2
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	TGGAACGTTTTCTACTTCAGG	47.6	42.9	57.3	55.8	597-740 bp	1.0	53.2
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	ATGGAACGTTTTCTACTTCAGGA	47.6	39.1	57.3	57.8	598-741 bp	1.0	52.7