Hotspot

rps16-trnQ-UUG

LSC · 6279-7261 · 983 bp

Back to Argentina

Pi 0.0563
MarkerSeek score 89.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
983
variable_sites
199
pi
0.0563
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.98
markerseek_score
89.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCGGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1009-1204 bp 1.0 91.2
rps16-trnQ-UUG_p2 GCGGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1007-1202 bp 1.0 91.2
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 991-1186 bp 0.933333 87.6
rps16-trnQ-UUG_p4 GATGTAAGACTCCACAGCGG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 1023-1218 bp 0.866667 85.5
rps16-trnQ-UUG_p5 GGATGTAAGACTCCACAGCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 1024-1219 bp 0.866667 85.5