Hotspot

ndhK

LSC · 51189-52067 · 879 bp

Pi 0.00282

MarkerSeek score 61.70

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 879
variable_sites: 6
pi: 0.00282
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	CGATCCTGCTGCATCTTCTT	GGGGGATGCTTGGTTACAAT	50.0	50.0	58.0	57.8	1073-1074 bp	1.0	79.2
ndhK_p2	AAACGATCCTGCTGCATCTT	GGGGGATGCTTGGTTACAAT	45.0	50.0	57.9	57.8	1076-1077 bp	1.0	78.7
ndhK_p3	AACGATCCTGCTGCATCTTC	GGGGGATGCTTGGTTACAAT	50.0	50.0	58.3	57.8	1075-1076 bp	1.0	76.9
ndhK_p4	CGATCCTGCTGCATCTTCTT	CATGGGGGATGCTTGGTTAC	50.0	55.0	58.0	58.6	1076-1077 bp	1.0	75.7
ndhK_p5	AAACGATCCTGCTGCATCTT	CATGGGGGATGCTTGGTTAC	45.0	55.0	57.9	58.6	1079-1080 bp	1.0	75.2