Hotspot

rps8-rpl14

LSC · 84024-84237 · 214 bp

Back to Arenga

Pi 0.00979
MarkerSeek score 63.90
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
214
variable_sites
6
pi
0.00979
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 CGGCAATATTGTCCCTACCC GAACTCGAGTTTTTGGTGCG 55.0 50.0 58.1 58.0 284-322 bp 1.0 84.5
rps8-rpl14_p2 CGGCAATATTGTCCCTACCC GCTCGGGAATTGAGACAGTT 55.0 50.0 58.1 57.9 261-299 bp 1.0 84.0
rps8-rpl14_p3 CGGCAATATTGTCCCTACCC ACTCGAGTTTTTGGTGCGAT 55.0 45.0 58.1 58.1 282-320 bp 1.0 83.7
rps8-rpl14_p4 CGGCAATATTGTCCCTACCC AACTCGAGTTTTTGGTGCGA 55.0 45.0 58.1 58.3 283-321 bp 1.0 82.0
rps8-rpl14_p5 CGGCAATATTGTCCCTACCC GGAACTCGAGTTTTTGGTGC 55.0 50.0 58.1 57.6 285-323 bp 1.0 81.5