Hotspot

rpl33-rps18

LSC · 71070-71424 · 355 bp

Back to Arenga

Pi 0.0413
MarkerSeek score 66.40
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
355
variable_sites
30
pi
0.0413
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.96
markerseek_score
66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 CAGATCTAGGGCAGGATCCA TGAGCTTGAGTTCCGATTGG 55.0 50.0 58.0 57.9 1198-1281 bp 1.0 85.3
rpl33-rps18_p2 AGATCTAGGGCAGGATCCAC TGAGCTTGAGTTCCGATTGG 55.0 50.0 58.0 57.9 1197-1280 bp 1.0 85.3
rpl33-rps18_p3 CAGATCTAGGGCAGGATCCA TAATCGATTCGATCCCCCGA 55.0 50.0 58.0 58.1 935-1015 bp 0.857143 79.9
rpl33-rps18_p4 AGATCTAGGGCAGGATCCAC TAATCGATTCGATCCCCCGA 55.0 50.0 58.0 58.1 934-1014 bp 0.857143 79.9
rpl33-rps18_p5 GGCAGGATCCACGAAATCAT TAATCGATTCGATCCCCCGA 50.0 50.0 58.0 58.1 926-1006 bp 0.857143 79.8