Hotspot

petA-psbJ

LSC · 65903-66933 · 1031 bp

Back to Arenga

Pi 0.011
MarkerSeek score 58.10
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1031
variable_sites
27
pi
0.011
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 AGGCCTTTTGCTCTTCTTGG GGTTCATATTCCGGGTTGGG 50.0 55.0 58.4 58.3 1161-1172 bp 1.0 76.8
petA-psbJ_p2 AGGCCTTTTGCTCTTCTTGG CATATTCCGGGTTGGGTTCA 50.0 50.0 58.4 57.6 1157-1168 bp 1.0 75.9
petA-psbJ_p3 AGGCCTTTTGCTCTTCTTGG GTTCATATTCCGGGTTGGGT 50.0 50.0 58.4 57.6 1160-1171 bp 1.0 75.9
petA-psbJ_p4 TGCTCTTCTTGGCATCTGTT GGTTCATATTCCGGGTTGGG 45.0 55.0 57.4 58.3 1153-1164 bp 1.0 75.3
petA-psbJ_p5 TTGCTCTTCTTGGCATCTGT GGTTCATATTCCGGGTTGGG 45.0 55.0 57.4 58.3 1154-1165 bp 1.0 75.3