Hotspot

psbM-trnD-GUC

LSC · 30986-31999 · 1014 bp

Back to Arenga

Pi 0.00507
MarkerSeek score 58.10
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1014
variable_sites
15
pi
0.00507
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAACAGTGCAGTAGC AGTTCAATCGGTCAGAGCAC 45.0 50.0 55.8 57.9 1114-1133 bp 1.0 63.5
psbM-trnD-GUC_p2 AGAATGAACAGTGCAGTAGC TATGCCATACACCTTGCTGG 45.0 50.0 55.8 57.7 1140-1159 bp 1.0 61.3
psbM-trnD-GUC_p3 AGAATGAACAGTGCAGTAGC CCATACACCTTGCTGGGATT 45.0 50.0 55.8 57.6 1136-1155 bp 1.0 60.6
psbM-trnD-GUC_p4 AGGAACTAGAATGAACAGTGCA AGTTCAATCGGTCAGAGCAC 40.9 50.0 57.4 57.9 1121-1140 bp 1.0 60.3
psbM-trnD-GUC_p5 AGAATGAACAGTGCAGTAGC CACCTTGCTGGGATTGTAGT 45.0 50.0 55.8 57.5 1131-1150 bp 1.0 60.2