Hotspot

petN-psbM

LSC · 30431-30880 · 450 bp

Back to Arenga

Pi 0.0117
MarkerSeek score 63.00
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
450
variable_sites
15
pi
0.0117
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.94
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 GTCTCACTTGGGCTGCTTTA TGCTACTGCACTGTTCATTCT 50.0 42.9 57.8 57.6 571-606 bp 1.0 73.3
petN-psbM_p2 ACTTGGGCTGCTTTAATGGT TGCTACTGCACTGTTCATTCT 45.0 42.9 57.7 57.6 566-601 bp 1.0 72.3
petN-psbM_p3 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTGTTCATTCT 50.0 42.9 57.6 57.6 516-551 bp 1.0 72.2
petN-psbM_p4 GTAAGTCTCACTTGGGCTGC TGCTACTGCACTGTTCATTCT 55.0 42.9 58.6 57.6 575-610 bp 1.0 70.3
petN-psbM_p5 TAAGTCTCACTTGGGCTGCT TGCTACTGCACTGTTCATTCT 50.0 42.9 58.6 57.6 574-609 bp 1.0 69.6