Hotspot

atpF-atpH

LSC · 14143-14687 · 545 bp

Back to Arenga

Pi 0.00606
MarkerSeek score 60.20
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
545
variable_sites
9
pi
0.00606
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 ATTAAACCCGAAACTCCCGG ATTTATGGACTGGTCGTGGC 50.0 50.0 57.9 58.0 661-705 bp 1.0 81.6
atpF-atpH_p2 CAGTGACCCAAGGAAAGGAA ATTTATGGACTGGTCGTGGC 50.0 50.0 57.3 58.0 633-677 bp 1.0 77.4
atpF-atpH_p3 CAGTGACCCAAGGAAAGGAAA ATTTATGGACTGGTCGTGGC 47.6 50.0 58.1 58.0 633-677 bp 1.0 74.2
atpF-atpH_p4 GTGACCCAAGGAAAGGAAAGA ATTTATGGACTGGTCGTGGC 47.6 50.0 57.8 58.0 631-675 bp 1.0 72.9
atpF-atpH_p5 CCAGTGACCCAAGGAAAGGA ATTTATGGACTGGTCGTGGC 55.0 50.0 59.2 58.0 634-678 bp 1.0 72.8