Hotspot

rps16

LSC · 4502-5556 · 1055 bp

Back to Arenaria

Pi 0.0756

MarkerSeek score 83.70

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1055
variable_sites: 118
pi: 0.0756
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GTTCAGGATCAGTCGTGGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.0	58.1	2279-2283 bp	0.666667	66.8
rps16_p2	GTTCAGGATCAGTCGTGGTC	AGACCGGTTCCAAATTCGAG	55.0	50.0	58.0	57.9	1810-1819 bp	0.666667	66.7
rps16_p3	AGGATCAGTCGTGGTCGTAT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	2275-2279 bp	0.666667	66.4
rps16_p4	AGGATCAGTCGTGGTCGTAT	AGACCGGTTCCAAATTCGAG	50.0	50.0	57.9	57.9	1806-1815 bp	0.666667	66.3
rps16_p5	GTTCAGGATCAGTCGTGGTC	GGTAAGGCATCGGGTTTTGA	55.0	50.0	58.0	58.2	2311-2315 bp	0.666667	66.1